Methods and Protocols

Quantitative PCR

Bacterial Transfection and Plasmid Prep

In-Vivo

• Tumor Initiation
• Orthotopic Lung Injections
• Treatment and Measurement
• Tumore Collection

Assays

• Enzyme-linked Immunosorbent Assay (ELISA)
• CXCL-10(human)
• IP-10(mouse)
• CSF-1(human)
• TGF-β2(human)
• IL-6(human)
• Luciferase reporter assay (Promega)
• Cyquant Growth Assay
• Clonogenic Growth Assay

Bioinformatics

Synthetic Lethal Screening

Statistical Analysis

Troubleshooting and Quality Assurance